G/ml Triphala for indicated time points were analyzed for DCF fluorescence (ROS generation) by flow cytometry after staining the cells with DCFDA. These experiments were repeated twice and obtained similar results. B) Activation of ERK, p53 and apoptosis by ROS can be abrogated by NAC. Cells were pretreated with 5 mM NAC for 1 hour and then treated with 60 g/ml Triphala for 4 h. p-ERK, p-p53 and c